Journal article
Glial Hedgehog signalling and lipid metabolism regulate neural stem cell proliferation in Drosophila
Q Dong, M Zavortink, F Froldi, S Golenkina, T Lam, LY Cheng
EMBO Reports | Published : 2021
Abstract
The final size and function of the adult central nervous system (CNS) are determined by neuronal lineages generated by neural stem cells (NSCs) in the developing brain. In Drosophila, NSCs called neuroblasts (NBs) reside within a specialised microenvironment called the glial niche. Here, we explore non-autonomous glial regulation of NB proliferation. We show that lipid droplets (LDs) which reside within the glial niche are closely associated with the signalling molecule Hedgehog (Hh). Under physiological conditions, cortex glial Hh is autonomously required to sustain niche chamber formation. Upon FGF-mediated cortex glial overgrowth, glial Hh non-autonomously activates Hh signalling in the N..
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Grants
Awarded by Australian Research Council
Funding Acknowledgements
We are grateful to Alex Gould, Thomas B. Kornberg, Isabel Guerrero, Yu Cai, William Chia, Tatsushi Igaki, Joseph M. Bateman, Kieran Harvey, Helena Richardson, Gary Hime and Philip Batterham for generous sharing of antibodies and fly stocks. We would like to thank Bloomington Drosophila Stock Center, Vienna Drosophila Resource Center, Fly Stocks of National Institute of Genetics, KYOTO Stock Center, Developmental Studies Hybridoma Bank and Addgene for fly stocks and plasmids. We would like to also thank OZDros for Drosophila quarantine, Peter MacCallum Cancer Institute Microscopy core and Biological Optical Microscopy platform at the University of Melbourne for technical assistance. We would like to thank Charles Robin and Mike Murray for sharing their microinjection facility with us. We are grateful to Kieran Harvey, Helena Richardson and Andrew Cox for critical reading of the manuscript. Schematic pictures in the figures are created with BioRender. QD is funded by a Melbourne Research Scholarship, LYC is funded by an ARC Future Fellowship, and LYC's laboratory is supported by funding from the NHMRC, ARC and the Peter MacCallum Cancer Foundation.